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Intra Cytoplasmic Sperm Injection This procedure allows us to fertilize an oocyte with only one single sperm cell. This is accomplished by maturing the oocyte until it has a polar body, purifying a sperm sample, injecting the sperm into the oocyte, culturing the injected egg until it divides (cleavage), surgically transferring the cleaved egg into the oviduct of the recipient mare. In the next pages, these separate steps will be illustrated. |