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Intra Cytoplasmic Sperm Injection

This procedure allows us to fertilize an oocyte
with only one single sperm cell.  This is
accomplished by maturing the oocyte until it
has a polar body, purifying a sperm sample,  
injecting the sperm into the oocyte,  culturing
the injected egg until it divides (cleavage),  
surgically transferring the cleaved egg into the
oviduct of the recipient mare.  In the next
pages, these separate steps will be illustrated.