 |
| |
 |
| |
 |
| |
 |
| |
 |
| |
 |
| |
 |
| |
 |
| |
 |
| |
 |
| |
 |
| |
 |
| |
 |
| |
 |
| |
 |
| |
 |
Rick Beck, D.V.M. 39185 Diamond Valley Rd Hemet, Ca 92543 888-4In-Foal office@infoalinc.com |
OOCYTE PREPARATION After the cumulus oophorus complex (COC) is flushed from the follicle, it must be cultured in a special culture media in an incubator rich in CO2 gas. Once it has finished the first phase on meiosis and expelled a polar body, it is ready to inject with a sperm cell. However, in order to inject it, the cells of the cumulus oophorus and the corona radiata must be removed. This is accomplished with and enzyme solution and mechanical manipulation with a small pipette. This procedure is called denuding. Click play to watch video below. |